|SOFT - TIAFT 1998||Scientific Session 2||Wednesday October 7, 1998|
|DETECTION TIMES OF 6-ACETYLMORPHINE, FREE MORPHINE AND TOTAL MORPHINE IN URINE AFTER SMOKING HEROIN|
|Michael L. Smith, Budda D. Paul, Eric Shimomura, Jacqueline Summers, Amanda J. Jenkins*, William D. Darwin* and Edward J. Cone*|
Division of Forensic Toxicology, Armed Forces Iinstitute of Pathology, Rockville, MD, USA.
Heroin was administered to four human subjects at doses of 3.5-13.9 mg by smoking. Urine samples were collected and tested by GC-MS procedures under blind conditions. The total amounts of 6-AM, free morphine and total morphine (M ± SEM) excreted in urine were 0.49 ± 0.14, 4.6 ± 1.0, and 53.5 ± 10.8 mole percent of the administered heroin, respectively. Detection times of the last specimen positive for the heroin metabolites at the cutoff concentrations indicated are presented in the table.|
Free morphine and total morphine at cutoff concentrations of 100 ng/mL and 2000 ng/mL, respectively, correlated better with 6-AM at a cutoff concentration of 10 ng/mL and produced fewer false positives and false negatives than total morphine at the cutoff concentrations of 300 or 4000 ng/mL. Measurement of total morphine and 6-AM required two confirmation methods. Measurement of free morphine had the advantage that it could be assayed with 6-AM in one confirmation procedure.