|SOFT - TIAFT 1998||Poster Session 2||Wednesday October 7, 1998|
WHIZZIES - A URINE DRUG SCREEN ADULTERANT|
Lisa D. Tarnai, Kurt L. Knachel, Clint R. Crooks
Scientific Testing Laboratories, Inc., 463 Southlake Blvd., Richmond VA 23236, USA
The internet and drug culture magazines tout nitrite containing products as a means of beating a urine drug test. Our study focuses on a product called "Whizzies", supplied in two vials containing sodium nitrite. The directions provided with the Whizzies state to "Add one vial to 45 - 60 mLs of urine." This results in a 16 to 21 mg/mL concentration of Whizzies in a urine sample. We performed studies to evaluate the effectiveness of Whizzies on urine containing free D9-carboxy-THC (THCA).
We added Whizzies in concentrations ranging from 2 mg/mL to 25 mg/mL to a series of human urine samples containing 75 ng/mL of THCA. The effect of Whizzies on Roche ONLINE and Diagnostic Reagents Incorporated (DRI) marijuana immunoassay reagents at 20 and 50 ng/mL cutoffs was determined. After four hours, all samples tested negative at the 50 ng/mL cutoff. The Roche Online reagents tested negative at 20 ng/mL when the Whizzies concentration exceeded 12.5 mg/mL. The DRI reagents tested negative at 20 ng/mL when the Whizzies concentration exceeded 4 mg/mL. All samples were analyzed by GC/MS. There was no recovery of free drug or internal standard in any of the samples.
Human urine samples containing Whizzies at 40 mg/mL and free THCA at concentrations ranging from 2 ng/mL to 500 ng/mL were analyzed with the Roche and DRI marijuana immunoassays using 20 and 50 ng/mL cutoffs. After two hours, the Roche ONLINE reagents tested positive at 50 ng/mL when the concentration of THCA was 250 ng/mL or greater. The Roche ONLINE reagents tested positive at 20 ng/mL when the concentration of THCA was 50 ng/mL or greater. The DRI reagents tested positive at 20 ng/mL when the concentration of THCA was 250 ng/mL or greater. All samples were analyzed by GC/MS. There was no recovery of free drug or internal standard in any of the samples.
This data demonstrates that Whizzies is effective at reducing the apparent free THCA concentration as measured by immunoassay and GC/MS. The effects of Whizzies on conjugated THCA will be covered.