SOFT - TIAFT 1998 Scientific Session 2 Wednesday October 7, 1998
Click Picture T.Z. Bosy, K.A. Cole

Division of Forensic Toxicology, Armed Forces Institute of Pathology, Washington, DC., USA

There has been a recent and significant increase in the use and availability of hemp seed oil products. When taken orally these products are being marketed as a healthy source of essential omega fatty acids. While the health aspects of these oils is open to debate, the probability that oils derived from the hemp seed will contain D9-tetrahyrdocannabinol (THC) is noteworthy. Recent additions to the literature site a number of studies illustrating that the ingestion of these products results in urinary levels of the THC metabolite, D9-tetrahydro-cannabinol carboxylic acid (THCA), well above the administrative cut-off (50ng/ml) used during random drug screens. The purpose of this study is to quantitate the THC levels in commercially available hemp oils and to administer those oils tested to THC-free volunteers to determine urine metabolite levels after a 15g dose. Two extraction protocols were evaluated for removing THC from the oil matrix; a single step liquid/liquid extraction was compared to a two-phase process using both liquid/liquid and solid phase techniques. Gas chromatograph/mass spectrometry was used to determine THC levels in several products; four Spectrum Essentials® (3 bottled oils and 1g capsules), two Health From the Sun® (1g capsules and bottled oil) oils, along with single samples of both Hempstead® and Hempola® hemp oils. These hemp oil products contained THC concentrations of 36.0, 36.4 117.5, 79.5; 48.6, 45.7; 21.0 and 19.2 µg/g respectively. FPIA and KIMS immunoassays were used to screen the urine samples and GC/MS was used to determine the amount of THC in each oil as well as confirm and quantitate THCA in the urine of study participants, immediately before and 6 hours after, each dose.

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