|SOFT - TIAFT 1998||Scientific Session 6||Thursday October 8, 1998|
|PROOF OF STEROID ABUSE BY DETECTION OF TESTOSTERONE ESTERS IN HAIR|
|Thieme, D., J. Grosse, R. K. Mueller, H. Sachs*|
Institute of Doping Analysis, Dresdner Str.12, Kreischa, Germany
|Two outstanding possibilities of hair analysis are access to retrospective information and identification of substances not excreted in urine. Both aspects are very promising for doping analysis, where the frequency and distribution of out-of-competition control can never be sufficient and exogenous abuse has to be distinguished from endogenous levels of identical substances (testosterone).
Logical scientific approaches for this differentiation (i.e. quantitation of T/E ratios or carbon isotope ratio discrimination) are always controversial due to common biological deviations. Therefore, the incorporation of anabolic agents - especially of exogenous precursors of anabolic steroids (steroid esters) was investigated. Extensive chromatographic procedures (HPLC clean-up, HRMS and MS/MS) were analytical prerequisites for successful sample preparation and identification due to low target concentration and interfering endogenous substances.
Five testosterone esters, nandrolone decanoate, metenolone enantate metandienone, stanozolol and clenbuterol could be identified in hair samples of 5 relevant forensic cases. The identification of 5 different testosterone esters enabled an unambiguous identification of testosterone abuse in three cases.
A comparison with analytical results obtained from urine and other biological tissues leads to conclusions about potential and limitations of steroid analysis in hair.