|SOFT - TIAFT 1998||Scientific Session 6||Thursday October 8, 1998|
|ANABOLIC STEROIDS IN HAIR OF 2 BODYBUILDERS|
|Pascal Kintz, Vincent Cirimele, Hans Sachs*, Bertrand Ludes|
Institut de Médecine Légale, 11 rue Humann, 67000 Strasbourg, France
|On March 1998, two tourists, coming from Spain by autocar were arrested by the French customs in Strasbourg. In their luggage, the custom-house officers discovered 2050 tablets of Winstrol (stanozolol, 2 mg) and Proviron (mesterolone, 25 mg) and 251 ampules of Winstrol Depot (stanozolol, 50 mg), Testoviron Depot (testosterone, 250 mg), Primobolan Depot (metenolone, 100 mg) and Deca-Durabolin (nandrolone decanoate, 50 mg). Both subjects claimed that the steroids were for personnal use, but this was not accepted by the officers, who considered the drugs as a part of trafficing. To document the pattern of use, the judge in charge of the case requested toxicological analyses, including urine and hair tests. Nandrolone, stanozolol, testosterone and their corresponding metabolites were identified in the urine of both subjects.
50 mg of hair were incubated in 1 ml of 1N NaOH at 95°C for 10 min, in presence of 5 ng of nandrolone-d3 and stanozolol-d3, used as internal standards. Then, the homogenate was extracted with 5 ml of ethyl acetate. After horizontal agitation and centrifugation, the organic phase was removed and evaporated to dryness. The residue was derivatized using 50 µl MSTFA/NH4I/2-mercaptoethanol (1000/2/5), for 20 min at 60°C. The drugs were identified by GC/MS in electron impact mode.
The hair tested positive for nandrolone (196 and 260 pg/mg), testosterone (46 and 71 pg/mg) and stanozolol (135 and 156 pg/mg), clearly indicating steroids abuse.
Although not yet recognized by the International Olympic Committee, in sports, hair analysis may be a useful adjunct to conventional drug testing in urine.