Department of Forensic Medicine, Akita University School of Medicine, Akita, JAPAN

Procedures
Powder of scilimurine containing 1% scilliroside was dissolved in 1% acetic acid solution. The mixture was applied to the Extrelut column, and was eluted with dichrolo-methane/iso-propanol (85:15 v/v). The eluent was evaporated completely and was dissolved again with mixture of methanol and an equal volume of phenacetin solution (0.2 mg/mL, I.S.) for HPLC analysis. The HPLC systems consisted of a LC-6A pump, Inertsil ODS-3V (4.6 mm x 150 mm) column and a diode array detector. Acetonitrile and water (23:77) were used for the mobile phase.

Results
The retention time of scilliroside was observed at 12.6 min (wavelength: 250 nm), and the internal standard was at 7.0 min (wavelength: 301 nm). This method can useful for determining scilliroside from biological materials such as blood and urine.