| SOFT - TIAFT 1998 | Poster Session 4 | Friday October 9, 1998 |
|
HPLC/MS/MS ANALYSIS OF FLUNITRAZEPAM, METABOLITES, AND OTHER BENZODIAZEPINES IN URINE AND WHOLE BLOOD Robert K. Lantz, Patricia L.Sulik, and Brian J. Kirby Rocky Mountain Instrumental Laboratories, 108 Coronado Ct., Ft. Collins, CO 80526, USA |
| Flunitrazepam (FNP), des-methyl-flunitrazepam, 7-amino-flunitrazepam, and 7-acetamido-flunitrazepam are analyzed by HPLC/MS/MS using a Waters C18 Symmetry column (3.9mm x 150mm), ammonium acetate buffer (pH 4.8, 40mM acetate, 40% v/v acetonitrile), 1.0mL/min. The internal standard is d5-nordiazepam. MS instrumentation includes Shimadzu LC10A/d pumps, P-E SciEx API-300 triple quadrupole mass spectrometer run in the MS/Ms mode, with SciEx MassChrom quantitation software. Ion transitions used are as follows: flunitrazepam (FNP): 314.2/205.1; 7-amino-FNP: 284.1/227.1; des-methyl-FNP: 300.1/254.1; 3-hydroxy-FNP: 330.1/284.1; 7-acetamido-FNP: 326.2/227.2; d5-nordiazepam: 276.1/213.0. Blood, standards, and hydrolyzed urine samples are mixed with internal standard, then extracted with n-butyl chloride. The organic layer is separated and dried under nitrogen. The organic layer is then reconstituted with mobile phase and analyzed in duplicate. LOD is less than 1 ng/mL for 14 benzodiazepines and metabolites tested. LOQ is 2 ng/mL for the same group of benzodiazepines and metabolites. |
 Previous Abstract |
Friday Index |
 Next Abstract |
Further information: tiaft98@aol.com or spiehleraa@aol.com
TIAFT & CBFT 1995 - 1997 All Rights Reserved