|SOFT - TIAFT 1998||Scientific Session 5||Thursday October 8, 1998|
|THE DETECTION OF 7-AMINOFLUNITRAZEPAM IN HAIR USING MICRO-PLATE ENZYME IMMUNOASSAY|
|Christine Moore1, Dawn Deitermann1, Douglas Lewis1, Robert Kronstrand2, Brian Feeley3, R. Sam Niedbala3|
1. U.S. Drug Testing Laboratories, IL, USA
Sexual abuse of both men and women while under the influence of so-called "date-rape" drugs has been the focus of many investigations. One of these drugs, flunitrazepam (Rohypnol, or "Roofies") has recently been banned in the U.S.A. because of its use in various "date-rape" situations. Unfortunately, the detection of flunitrazepam or two of its metabolites, desmethyl-flunitrazepam and 7-aminoflunitrazepam, (7-AF) in a single specimen such as urine or blood is difficult in criminal situations because of the likelihood of single dose ingestion and the length of time since the alleged incident. Hair provides a solution to the second of these problems, in that drugs tend to incorporate into hair and remain there for longer periods of time than either urine or blood. The objective of this paper was to determine whether a commercially available micro-plate enzyme immunoassay system was sufficiently sensitive for the routine screening of 7-AF in hair.