|SOFT - TIAFT 1998||Poster Session 4||Friday October 9, 1998|
COMPARISON OF THREE DIFFERENT DIGESTION PROCEDURES FOR SYSTEMATIC TOXICOLOGICAL ANALYSIS IN TISSUES|
Teresa Soriano, Carmen Jurado, Manuel Menéndez, Manuel Repetto
Instituto Nacional de Toxicología, P.O. Box 863, 41080 Sevilla, Spain
|In a forensic toxicological laboratory the analysis of tissues, such as liver, kidney, brain, etc., is usually required and necessary. In order to achieve a suitable and reliable work-up and extraction method, three different digestion procedures were compared. Two of them used solvent digestion with methanol or phosphate buffer pH 6, followed by sonication and centrifugation. The other used an enzymatic hydrolysis with proteinase K at pH 7, which avoids the decomposition of labile drugs under drastic conditions. The extracts obtained after the above mentioned procedures were subjected to our previously developed SPE method.
The experiment was performed on real samples from cadavers which previously tested positive for different compounds in their respective biological fluids.
After comparison, the enzymatic method gave the cleanest extracts and the highest relative recoveries for all the compounds tested.