Institute of Forensic Medicine, Aachen University of Technology, 52057 Aachen, Germany

0.5-1.5 ml aliquots of biological fluids were spiked with appropriate deuterated internal standards and extracted using a common solid phase extraction method (C-18 cartridges). The extracts were subjected to liquid chromatographic-atmospheric pressure chemical ionization mass spectrometric examination (positive ions), using selected ion monitoring procedures. These procedures were developed after analysis of full-scan mass spectra of examined compounds. The extraction method appeared very universal; the recoveries ranged from 73% to 98% for all drugs but EME (41%), the LOD's ranged from 0.1 to 2.5 µg/ for all drugs but C6G (100 µg/l). Overall precision was 2-11% RSD. The extracts analyzed by HPLC-DAD and full-scan GC-MS appeared very clean. The procedure was applied for routine forensic casework.