|SOFT - TIAFT 1998||Poster Session 4||Friday October 9, 1998|
DETERMINATION OF COMMON DRUGS OF ABUSE IN BODY FLUIDS USING ONE ISOLATION PROCEDURE AND LIQUID-CHROMATOGRAPHY-ATMOSPHERIC PRESSURE CHEMICAL IONIZATION MASS SPECTROMETRY|
Maciej J.Bogusz, Rolf-Dieter Maier, Klaus-Dieter Krüger and Ulrike Kohls
Institute of Forensic Medicine, Aachen University of Technology, 52057 Aachen, Germany
|The method of determination of opiate agonists (morphine, morphine-3-glucuronide, morphine-6-glucuronide, 6-monoacetylmorphine, codeine, codeine-6-glucuronide, dihydrocodeine, dihydromorphine, buprenorphine, methadone, tramadol), cocaine and its metabolites (benzoylecgonine and ecgonine methyl ester), ibogaine and lysergic acid diethylamide in serum, blood, urine and other biological matrices is presented.
0.5-1.5 ml aliquots of biological fluids were spiked with appropriate deuterated internal standards and extracted using a common solid phase extraction method (C-18 cartridges). The extracts were subjected to liquid chromatographic-atmospheric pressure chemical ionization mass spectrometric examination (positive ions), using selected ion monitoring procedures. These procedures were developed after analysis of full-scan mass spectra of examined compounds. The extraction method appeared very universal; the recoveries ranged from 73% to 98% for all drugs but EME (41%), the LOD's ranged from 0.1 to 2.5 µg/ for all drugs but C6G (100 µg/l). Overall precision was 2-11% RSD. The extracts analyzed by HPLC-DAD and full-scan GC-MS appeared very clean. The procedure was applied for routine forensic casework.