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| Monday, August 25 | Postmortem Analysis and Drug Stability |
SUPERCRITICAL FLUID EXTRACTION AS A SAMPLE PREPARATION TECHNIQUE FOR THE DETERMINATION OF MORPHINE AND 6-MONOACETYL-MORPHINE IN POSTMORTEM BLOOD AND VITREOUS HUMOUR
Scott K.S., Oliver J.S. The use of supercritical fluid extraction (SFE) was investigated for the detection of heroin abuse by quantifying levels of morphine and 6-monoacetyl-morphine (6-MAM) in post-mortem blood and vitreous humor samples. The levels achieved in each of the two toxicological specimens were compared on a case to case basis to determine if a correlation exists. Blood/vitreous humor samples containing deuterated morphine as the internal standard, were loaded on Extrelut®, dried at room temperature and then extracted using supercritical carbon dioxide:methanol:triethylamine (90:8.5:1.5) at 100° C and 3500 psi for 20 minutes. The collected extracts were analysed by GC/MS. Results show that, in general, the levels of morphine obtained from blood are higher than the corresponding levels in vitreous humor and that correlation between the two sample types is good. Preliminary studies for 6-MAM again show lower levels in vitreous humor than in blood, with the levels being lower than the corresponding morphine levels in both cases.
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