Smysl B.^*, Sevcik J.^**,****, Lemr K.^**,****, Vanak J.^***, Adam T.^****

^* Institute of Forensic Medicine and Medical Law, Palacky University, Hnevotinska 3, 775 09 Olomouc, Czech Republic
^** Department of Analytical Chemistry, Palacky University, Tf. Svobody 8, 771 26 Olomouc, Czech Republic
^*** Exbio src, Hnevotinska 52, 779 00 Olomouc, Czech Republic
^**** Laboratory of Bioanalytical Research, Palacky University, Tf. Svobody 8, 771 26 Olomouc, Czech Republic

Present population, which is subject to a growing influence of drugs, calls for wider control of their abuse in the environment (e.g. ambulancy, policy, drop-in centers). Immunochemical tests have proved convenient for this control, mainly because of their simplicity, speed of determination and the possibility of running analysis of larger sample series at relatively lower costs. Independent analytical methods (e.g. GC, HPLC, HPCE) then have to be applied for verification of these tests.
Presented poster describes immunochemical tests based on polyclonal antibodies and capillary electrophoresis methods for the determination of individual groups of widespread drugs - amphetamines, benzodiazepines, cannabinoids, cocaine and opiates. The applicability of prepared antibodies for selective screening of mentioned drug groups is demonstrated both on a wide set of model and real samples of biological fluids (urine, salivas). The abilities of the use of capillary electrophoresis for the identification of concrete abused drugs and/or their main metabolites, respectively methamphetamine (amphetamine), flunitrazepam (7-aminoflunitrazepam), δ⁹-tetra-hydrocannabinol (11-nor-δ⁹-tetrahydrocannabinol acid), cocaine (benzoyl-ecgonine) and heroin (morphine) are shown. The advantage of application of capillary electrophoresis in comparison to GC and HPLC as final verification tools can be seen in variability of its operation modes and short analysis time.

Finantial support of this work by the grant of MSMT VS 96021, grant UP Olomouc 14201101 and Exbio, src Olomouc is gratefully acknowledged