TIAFT Young Scientists "Workshop 2001"

Determination of Cocaine and its Metabolites in Saliva by GC/MS-EI

P. Càmpora O.; A.M. Bermejo B.; M.J. Tabernero and P. Fernàndez

Institute of Legal Medicine. University of Santiago of Compostela. Faculty of Medicine. San Francisco s/n. Santiago of Compostela. Spain

A simple analytical method for the simultaneous determination of Cocaine and its main metabolites, ecgonine methyl ester (EME) and benzoylecgonine (BEG) in saliva was developed. A procedure of extraction liquid-liquid with Toxilab (distributed by Dipesa, Spain), derivatization with N, O-bis-trimethylsilyl-trifluoroacetamide (BSTFA) and trimethylchlorosilane (TMCS, 1%), and subsequent analysis by gas chromatography/ mass spectrometry (GC/MS) in SIM mode have been carried out.

The answer of the detector was linear in a concentration range of 25-1000 ng/ mL for each analyte, with a reproducibility of 4.33% for EME, 9.55% for Cocaine and 5.49% for BEG, and a recovery of extraction of 99.15%, 98.78% and 106.97%, for EME, Cocaine and BEG, respectively, at a concentration of 300 ng/mL.

The limits of detection (LOD) were 1.7 ng/mL for EME, 0.2 ng/mL for Cocaine and 4.2 ng/mL for BEG and the limits of quantification (LOQ) were 5.7 ng/mL for EME, 0.8 ng/mL for Cocaine and 14.1 ng/mL for BEG.

Finally, the method was applied to 40 real samples of saliva, collected with Salivette (Sarstedt), 24 of them positive for cocaine, 18 for BEG and 12 for EME. In view of the obtained results, we concluded that the saliva is an alternative biological sample for the determination of cocaine and their metabolites.

ABSTRACTS

• Pitfalls associated with the use of a simple sample preparation in the analysis of saliva with LC-ESI-MS/MS.
  K.A. Mortier, K.M. Clauwaert, W.E. Lambert, J.F. Van Bocxlaer, E.G. Van den Eeckhout, C.H. Van Peteghem, and A.P. De Leenheer
   
  
• Oral Fluid Testing in Road Traffic - The German Experience within the ROSITA Project
  S. Steinmeyer, H. Ohr, and M. R. Moeller
   
  
• The use of oral fluid and sweat wipes for the detection of drugs of abuse in impaired drivers
  Nele Samyn, Bart Viaene, Bart Laeremans, Gert De Boeck
   
  
• Detection of Fluoxetine and Norfluoxetine in Saliva
  Fiona M. Wylie and John S. Oliver
   
  
• Measurement of Amphetamines in Biological Samples by LC-MS/MS
  Michelle Wood, Michael Morris, Donald P Cooper
   
  
• Immunochromatographic teststrips for saliva analysis
  Jörg Zimmermann1, Nele Samyn, Maria da Costa, Thomas Wuske, Rainer Polzius, Hans-Jürgen Duchstein1
   
  
• Determination of MDMA ('Ecstasy') in saliva: a controlled study in recreational users
  Monica Navarro, Magi Farré, Simona Pichini, Jordi Ortuño, Pere Nolasc Roset, Ester Menoyo, Jordi Segura, Rafael de la Torre
   
  
• Determination of MDMA ('Ecstasy') in saliva with Drugwipe analytical device: a controlled study in recreational users
  Monica Navarro, Magi Farré, Simona Pichini, Piergiorgio Zuccaro, Roberta Pacifici, Jordi Ortuño, Pere Nolasc Roset, Ester Menoyo, Jordi Segura, Rafael de la Torre
   
  
• Elimination kinetics of oral nandrolone in urine and saliva
  V. Cirimele, P. Kintz B. Ludes
   
  
• Determination of Cocaine and its Metabolites in Saliva by GC/MS-EI
  P. Campora O.; A.M. Bermejo B.; M.J. Tabernero and P. Fernandez.
   
  
• The Finnish experience from the ROSITA project
  Marielle Grönholm and Pirjo Lillsunde